ROTHMAN, JAMES, Ph.D. Professor of Physiology & Cellular Biophysics Adjunct Professor, Department of Physiology and Cellular Biophysics Columbia University Chairman Department of Cell Biology Wallace Professor of the Biomedical Sciences, and Professor of Chemistry Yale University More Info >>

Current Research

(See Rothman Lab; and Columbia Genome Center)

1) ELUCIDATING THE UNDERLYING MECHANISMS OF TRANSPORT WITHIN CELLS

2) HIGH THROUGHPUT CELL BIOLOGY

Selected Publications

Intaek Lee, Neeraj Tiwari, MyunHwa Dunlop, Morven Graham, Xinran Liu, and James E. Rothman. 2014, Membrane Adhesion Dictates Golgi Stacking and Cisternal Morphology, Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1849-54. doi: 10.1073/pnas.1323895111. Epub 2014 Jan 21. PMID:24449908, PMCID: PMC3918774 [Available on 2014/8/4]

Li F, Kummel D, Coleman J, Reinisch KM, Rothman JE and Pincet F. A Half-Zippered SNARE Complex Represents a Functional Intermediate in Membrane Fusion. J Am Chem Soc. 2014 Mar 5;136(9):3456-64. doi: 10.1021/ja410690m. Epub 2014 Feb 18. PMID: 24533674 PMCID:PMC3985920 [Available on 2015/1/23]

Richard W. Cho, Daniel Kummel, Feng Li, Stephanie Wood Baguley, Jeff Coleman, James E. Rothman, and J. Troy Littleton. Genetic Analysis of the Complexin Trans-clamping Model for Cross-linking SNARE Complexes In Vivo, (2014) Proc Natl Acad Sci USA. Published ahead of print June 30, 2014, doi:10.1073/pnas.1409311111

Lavieu. G., Dunlop, MH., Lerich, A., Zheng H., Bottanelli, F., Rothman J.E. (2014) The Golgi-Ribbon Structure Facilitates Anterograde Transport of Large Cargoes. MBoC doi:10.1091/mbc.E14-04-0931

Sylvain Zorman, Aleksander A Rebane, Lu Ma, Guangcan Yang, Matthew A Molski, Jeff Coleman, Frederic Pincet, James E Rothman, Yongli Zhang (2014) Common intermediates and kinetics, but different energetics, in the assembly of SNARE proteins DOI: http://dx.doi.org/10.7554/eLife.03348. eLife 2014;10.7554/eLife.03348.

Wang J, Bello O, Auclair SM, Wang J, Coleman J, Pincet F, Krishnakumar SS, Sindelar CV & Rothman JE. Calcium-Sensitive Ring-like Oligomers Formed by Synaptotagmin PNAS 2014; Sept. 23;111(38): 13966-12971, doi:10.1073/pnas.1415849111.

Yamamoto, A. et al. 2006. Autophagy-mediated clearance of huntingtin aggregates triggered by the insulin-signaling pathway. J. Cell Biol. 172, 719-31.

Antinozzi, P.A. et al. 2006. Functional mapping of disease susceptibility loci using cell biology. PNAS 103, 3698-703.

Giraudo, C.G. 2006. et al. A clamping mechanism involved in SNARE-dependent exocytosis. Science 313, 676-80.

Becker, T., Volchuk, A., Rothman, J.E. 2005. Differential use of endoplasmic reticulum membrane for phagocytosis in J774 macrophages. Proc Natl Acad Sci U S A 102, 4022-6.

Cosson, P. et al. 2005. Dynamic transport of SNARE proteins in the Golgi apparatus. Proc Natl Acad Sci U S A 102, 14647-52.

Paumet, F., Rahimian, V., Di Liberto, M., and Rothman, J.E. 2005. Concerted auto-regulation in yeast endosomal t-SNAREs. J Biol Chem 280, 21137-43.

Giraudo, C.G. et al. 2005. SNAREs can promote complete fusion and hemifusion as alternative outcomes. J. Cell Biol. 170, 249-60.

Fix, M. et al. 2004. Imaging single membrane fusion events mediated by SNARE proteins. Proc Natl Acad Sci U S A 101, 7311-6.

Fukasawa, M., Varlamov, O., Eng, W.S., Sollner, T.H., 2004. Rothman, J.E. Localization and activity of the SNARE Ykt6 determined by its regulatory domain and palmitoylation. Proc Natl Acad Sci U S A 101, 4815-20.

Paumet, F., Rahimian, V., and Rothman, J.E. 2004. The specificity of SNARE-dependent fusion is encoded in the SNARE motif. Proc Natl Acad Sci U S A 101, 3376-80.

Varlamov, O. et al. 2004. i-SNAREs: inhibitory SNAREs that fine-tune the specificity of membrane fusion. J Cell Biol 164, 79-88.

Volchuk, A. et al. 2004. Countercurrent distribution of two distinct SNARE complexes mediating transport within the Golgi stack. Mol Biol Cell 15, 1506-18.

Hu, C. et al. 2003. Fusion of cells by flipped SNAREs. Science 300, 1745-9.

Recent Honors

Officer of the Legion of Honor (Republic of France) 2014

Dr.honoris causa, École Normale Supérieure (France) 2014

Major Grants

Wellcome Trust Strategic Award / Nanoscopy of Dynamics in the Living Cell

NIH/NIDDK / Mechanism of Membrane Fusion by SNARE Proteins

NIH/NIGMS / Regulation of Exocytosis at Neuronal Synapses

NIH/NIGMS / Cortical ER Biogenesis in Mammalian Cells